MTBN Purity Assessment Kit

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Catalog Numbers 020033 (32 reactions) and 020032 (96 reactions)

Accumol's MTBN Purity Assessment Kit allows the detection of Monocyte, T-Cell, B-Cell and Neutrophil mRNA in genomic DNA samples prepared from sorted Cells. This kit is designed to detect and quantify the lineage-specific mRNAs that are co-purified along with genomic DNA in standard DNA isolation procedures. The kit is primarily intended to assess the purity of Myeloid cell preparations (for example CD14+, CD15+, CD66b, CD33+ cells), as well as lymphoid cell preparations (T or B cells). Because it will detect the most abundant contaminating cell types, the kit can also be used to estimate the purity of other cell preparations, such as NK cells.
After reverse transcription and PCR amplification, the reaction products are analyzed by fragment analysis on a sequencer. Alternatively, analysis can be performed on agarose or polyacrylamide gel.

Since the test is performed on isolated nucleic acids, Accumol's MTBN Purity Assessment Kit is not affected by the presence of beads or antibodies on the surface of the sorted cells, and can be performed regardless of the sorting method used (flow cytometry, magnetic beads, density-based, adhesion, etc...). And because the kit detects the purity information directly at the nucleic acid level, the sample can be analyzed at any point in time, even months after the actual cell sorting took place.

Easy to use

The MTBN Purity Assessment Kit is provided ready to use as a reaction mix aliquoted in 0.2 mL PCR tubes. Just add the template (10 ng of gDNA sample, or 5 ng of RNA), and load the reaction tube in your thermocycler.

PCR products analysis is preferentially performed on a sequencer, using conditions similar to the ones used in most chimerism analysis procedures.

Alternatively, PCR products can be analyzed by electrophoresis on agarose or polyacrylamide gel. However, the sensitivity and dynamic range will be significantly reduced as compared to fragment analysis on a sequencer.

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Benefits

  • Assess the purity of sorted cells directly in the genomic DNA sample.
  • Save cells; stop performing flow cytometry
  • Works with as little as 10 ng of DNA
  • Just add sample to pre-filled reaction tube
  • Use the equipment already in your lab
  • Easy integration within existing workflow
  • Can be performed years after cell sorting

Applications