Non-Myeloid Genomic Detection Kit

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Catalog numbers:

No Passive Reference: 030062 (96 reactions), 030063 (32 reactions)
Low Passive Reference: 030072 (96 reactions), 030073 (32 reactions)
Passive Reference: 030082 (96 reactions), 030083 (32 reactions)

Accumol's qPCR Non-Myeloid Genomic Detection Kit allows the detection of T-Cell and B-Cell DNA in genomic DNA samples prepared from sorted Myeloid Cells. During normal T- and B- Cell differentiation, DNA rearrangement takes place on the TCR and IGH loci, respectively. The qPCR Non-Myeloid Genomic Detection Kit is designed to detect these rearrangements. The detection of the target sequences in the DNA sample indicates that contaminating T- and/or B-Cells were present in the original Myeloid Cell preparation. Following PCR, the amplification products are analyzed on a qPCR* instrument by melting curve analysis.

Since the test is performed on DNA, Accumol's qPCR Non-Myeloid Genomic Detection Kit is not affected by the presence of beads or antibodies on the surface of the sorted cells, and can be performed regardless of the sorting method used (flow cytometry, magnetic beads, density-based, adhesion, etc...). And because Accumol's qPCR Non-Myeloid Genomic Detection Kit detects the purity information directly at the level of the DNA sample, the sample can be analyzed at any point in time, even years after the actual cell sorting took place.

*quantitative PCR, also called real-time PCR, RT-PCR

Easy to use

The qPCR Non-Myeloid Genomic Detection Kit is provided as a reaction mix ready to be dispensed in qPCR tubes or plates. Just add the template (about 10 ng of gDNA), and load the reaction tubes or plates in your qPCR instrument.

If your laboratory does not have access to a qPCR instrument, please use the MTBN Purity Assessment kit, designed to check the purity of Myeloid cells using classical PCR and fragment analysis on a sequenceur.

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Benefits

  • Assess the purity of Myeloid cells directly in the genomic DNA sample.
  • Save cells; stop performing flow cytometry
  • Works with as little as 10 ng of DNA
  • Just add sample to ready-to-use mix
  • Use the equipment already in your lab
  • Easy integration within existing workflow
  • Can be performed years after cell sorting

Applications