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Should you require help or technical information about our products or protocols, please contact our technical support department at support@accumol.com



Technical Documentation

Non-T Genomic Detection Kit PDF in letter or A4 format.

Non-B Genomic Detection Kit PDF in letter or A4 format.

MTBN Purity Assessment Kit PDF in letter or A4 format.

qPCR Non-T Genomic Detection Kit PDF in letter or A4 format.

qPCR Non-B Genomic Detection Kit PDF in letter or A4 format.

qPCR Non-Myeloid Genomic Detection Kit PDF in letter or A4 format.



Material Safety Data Sheets (MSDSs)

Non-T Genomic Detection Kit:

US and Canada PDF

Europe PDF

Non-B Genomic Detection Kit:

US and Canada PDF

Europe PDF

MTBN Purity Assessment Kit:

US and Canada PDF

Europe PDF

qPCR Genomic Detection Kits:

US and Canada PDF

Europe PDF



Fluorescence Adjustment Factor

Lot 522401: 0.6

Lot 530201: 1.1

Lot 612001: 0.9

Lot 624401: 0.8

Lot 629301: 0.54

Lot 710901: 0.62

Lot 717001: 1.0

Lot 807801: 0.76

Lot 824701: 0.62

Lot 835101: 0.67

MTBN Purity Assessment KIt and qPCR Kits: contact us



Certificate of Analysis

Lot:522401 PDF

Lot:526101 PDF

Lot:530201 PDF

Lot:612001 PDF

Lot:624401 PDF

Lot:629301 PDF

Lot:710901 PDF

Lot:717001 PDF

Lot:734801 PDF

Lot:807801 PDF

Lot:824701 PDF



Frequently Asked Questions

Non-T and Non-B Genomic Detection Kits


Q: Is my current sorting method compatible with these kits?

A: Yes! The Non-T Genomic and Non-B Genomic Detection Kits determine cell purity at the nucleic acid level. It is therefore insensitive to the method used to sort the cells.

Q: Do I need to introduce a new technique and procedures in the lab to use these kits?

A:These kits are designed to be used along the traditional chimerism analysis workflow. All there is to do is add the DNA sample to the reaction tube. Then the reaction can be run at the same time than the chimerism PCR, because the reaction parameters are the same. The PCR products are analyzed on a sequencer in the same way than the chimerism sample. Chimerism labs already have all the expertise and equipment to run the Non-T Genomic and Non-B Genomic Detection Kits.

Q: I don't have a lot of DNA sample to start with, I need it all for the chimerism analysis and cannot waste any. Will I have enough sample to check for purity?

A:The Non-T Genomic and Non-B Genomic Detection Kits can be run with as little as 0.5 ng of genomic DNA. This corresponds to the amount of DNA present in approximately 200 cells. The lowest DNA concentration that can be detected with classical UV methods is about 2 ng/µL. So even with samples with very low, non detectable DNA content, 1 µL of sample is usually sufficient to run the Genomic Detection Kit.

Q: I validated my cell sorting method and had good purities. Why would I need to check the purity of the sorted cells every time?

A:The quality of the cell sorting is very sample-dependant. The first source of viability is of course the patient. Shortly after hematopoietic stem cell transplant, it is common for patients to have a very low blood cell count, as well as a very low T cell count (often less than 5% of total leukocytes, whereas normal levels are 25-30%). The risk of T Cells isolated from these samples to be contaminated by monocytes or granulocytes is very high.

In addition, blood samples sometimes reach the lab several days after collection, and can be exposed to extreme temperatures during transportation. These parameters can vary between samples, are out of the lab's control, and can have a very negative impact on cell sorting quality.

Assessing the purity of each isolated cell subset is an essential quality control step, and is required for ASHI or EFI accreditation.

Q: Isn't flow cytometry a more accurate method of assessing purity?

A: Actually, flow cytometry is not as accurate as one might think, because measured purity can vary considerably depending on the way the data is analyzed. On most samples, changing the way the cells are gated can lead to purities readings anywhere between 80% and 100%. In contrast, calculating purity is straightforward with the Non-T Genomic and Non-B Genomic Detection Kits, and there is no interpretation or ambiguities during the analysis.

In addition, chimerism tests are based on DNA analysis, and so are the Non-T Genomic and Non-B Genomic Detection Kits. All the DNA present in the sample will be taken into account. In contrast, flow cytometry analysis excludes "debris" and events that have a small size. These may contain DNA fragments or even entire nuclei. This DNA, excluded from the flow cytometry data, might have an impact on the chimerism analysis results.



Ordering and Shipping


Q: I would like to try out one of your products can I get a sample?

A:Yes! Absolutely! New customers can request a sample by simply filling out our sample order form. We ship the sample to you at no cost.

Q: Do you ship internationally?

A:Yes! Absolutely! Please contact us for specific shipping costs at orders@accumol.com .

Q: How do I find out about pricing in my country?

A:Just contact us at orders@accumol.com in order to receive detailed pricing for products and shipping charges to your country.

Quick Links

 

News

qPCR kits released!
April 25, 2017
The Non-T and Non-B Genomic Detection kits are now available for quantitative PCR (real-time PCR) instruments. In addition, we are pleased to introduce the brand new qPCR Non-Myeloid Genomic Detection kit, designed to assess the purity of Myeloid cell preparations directly at the DNA level.

New Publication
January 2015
British Journal of Haematology, 2015, 168, 26–37

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