Fragment Analysis Settings

After PCR amplification, the Non-T and Non-B Genomic Detection kits amplification products are analyzed by capillary electrophoresis on a sequencer (genetic analyser).

Instrument Settings

On Applied Biosystems instruments (such as the 3500, 3730, SeqStudio Genetic Analysers), the settings used with the chimerism/identity testing kit are usually compatible with Accumol's kits. The kits amplification products are labeled with 6-FAM. Typically, choosing an internal size standard labeled with LIZ (with filter set G5) or ROX (with filter set D) will give good results.

Sample Preparation

Mix 0.5µL of amplification product, 0.5 µL of internal size standard, 9 µL of formamide. Denature 3 minutes at 95ºC, then immediately transfer on ice. Keep on ice until ready to load the sequencer.

GeneMapper panel and bin files

These files can be imported into GenMapper 4.1 or newer versions, as well as into the Microsatellite Analysis (MSA) online application on the ThermoFisher cloud. Since the calculated size of the amplicons can vary slightly between instruments and experimental conditions, the bin and panel sizes might need to be adjusted to ensure proper peak identification.

Non-T Genomic Detection Kit:
Panel file
Bin file

Non-B Genomic Detection Kit:
Panel file
Bin file

qPCR Settings

The typical settings for Applied Biosystems QuantStudio instruments are shown below. For other instruments, please contact technical support at support@accumol.com.

6-FAM, GeneMapper, SeqStudio, QuantStudio, are registered trademarks of Applied Biosystems Inc